Sumários

Intermediate Purification: Precipitation

16 Maio 2019, 10:00 Ana Margarida Nunes da Mata Pires de Azevedo

Intermediate Purification.

Precipitation. Types of precipitation: i) pH adjustment; ii) selective denaturation; iii) Addition of precipitants. Selective denaturation: temperature, pH and organic solvents. Addition of precipitant agents: salts, non-ionic polymers, organic solvents, polyelectrolytes and affinity ligands. Salting out and the Cohn equation.

Kahoot quiz.


Lab 6. Mass and Energy Balances for the Production of PGA - I

15 Maio 2019, 13:30 Ana Margarida Nunes da Mata Pires de Azevedo

Calculation session for mass and energy balances regarding a penicillin G acylase production process, built over the collected laboratory data.

Part I: set-up of a unit operations flow sheet for the process; selection of data; and establishment of the balance equations up to the fermentation step. 


Membranes Processing

15 Maio 2019, 11:00 Ana Margarida Nunes da Mata Pires de Azevedo

Mathematical description of membrane processes. Definition of transmembrane pressure, rejection coefficient and transmission coefficient. Modes of operation: i) clarification; ii) concentration; iii) diafiltration. Modelling of membrane processes: i) Pore model; ii) Resistance model; iii) Mass transfer model; iv) Osmotic pressure model.


Lab 6. Mass and Energy Balances for the Production of PGA - I

13 Maio 2019, 10:00 Ana Margarida Nunes da Mata Pires de Azevedo

Calculation session for mass and energy balances regarding a penicillin G acylase production process, built over the collected laboratory data.

Part I: set-up of a unit operations flow sheet for the process; selection of data; and establishment of the balance equations up to the fermentation step. 


Introduction to membrane processes

9 Maio 2019, 10:00 Ana Margarida Nunes da Mata Pires de Azevedo

Definition of a membrane. Driving forces: hydrostatic pressure, concentration, electrical potential and temperature gradients. Membrane separations by hydrostatic pressure: microfiltration, ultrafiltration, nanofiltration, reverse osmosis. Types of membranes: i) chemical characteristics (polymeric, inorganic and metallic membranes); ii) Morphologic characteristics (presence of pores; degree of symmetry; pore size); iii) module configuration (planar versus tubular membranes). Examples of symmetric, asymmetric and composite membranes.

Membrane modules: i) flat sheet membranes (plate and frame, spiral wound), ii) Tubular membranes (tubular, capillary and hollow fibers). Membranes fabrication.

Membrane processes: applications. Design considerations: dead-end versus tangential-flow filtration. Membrane clogging: Concentration polarization versus fouling.

Kahoot quiz.