Balanços de Massa e Energia para a Produção de PGA - II

22 maio 2019, 13:30 • Ana Margarida Nunes da Mata Pires de Azevedo

Continuação da sessão de cálculos de balanços de massa e energia para um processo de produção de PGA.

Parte II: recuperação das células; selecção da centrifuga; lavagem das células; Choque osmótico.

Intermediate Purification: liquid-liquid extraction

22 maio 2019, 11:00 • Ana Margarida Nunes da Mata Pires de Azevedo

Types of liquid-liquid extraction: i) extraction with organic solvents, ii) extraction with reversed micelles; iii) extraction with aqueous two-phase systems, iv) extraction with supercritical fluids. Extraction parameters: partitioning coefficient, extraction factor, extraction yield, selectivity and concentration factor. Batch extraction versus continuous extraction. Single stage extraction versus multistage extraction. Extraction equipment: Mixer-settler, static columns, pulse columns, agitated columns (rotary columns and vibrating plates column), centrifugal extractors.

Balanços de Massa e Energia para a Produção de PGA - II

20 maio 2019, 10:00 • Ana Margarida Nunes da Mata Pires de Azevedo

Continuação da sessão de cálculos de balanços de massa e energia para um processo de produção de PGA.

Parte II: recuperação das células; selecção da centrifuga; lavagem das células; Choque osmótico.

Intermediate Purification: Precipitation

16 maio 2019, 10:00 • Ana Margarida Nunes da Mata Pires de Azevedo

Intermediate Purification.

Precipitation. Types of precipitation: i) pH adjustment; ii) selective denaturation; iii) Addition of precipitants. Selective denaturation: temperature, pH and organic solvents. Addition of precipitant agents: salts, non-ionic polymers, organic solvents, polyelectrolytes and affinity ligands. Salting out and the Cohn equation.

Kahoot quiz.

Lab 6. Mass and Energy Balances for the Production of PGA - I

15 maio 2019, 13:30 • Ana Margarida Nunes da Mata Pires de Azevedo

Calculation session for mass and energy balances regarding a penicillin G acylase production process, built over the collected laboratory data.

Part I: set-up of a unit operations flow sheet for the process; selection of data; and establishment of the balance equations up to the fermentation step.