30 Maio 2019, 10:00 • Ana Margarida Nunes da Mata Pires de Azevedo
Separations based on size: Size-Exclusion chromatography. Principles of separation. Commonly used stationary phases.
Separations based on affinity interactions: Affinity chromatography. Examples of biospecific interactions: sugars-lectins, enzyme-substrates (analogues, inhibitors, co-factors), antibodies-antigens, antibodies-protein A/G/L. Pseudo-affinity chromatography: example of multimodal ligands, dye-ligands, immobilized metal-ligands, biomimetic ligands.