Sumários
2-DE gel analysis using the Samespots software
22 novembro 2019, 11:00 • Miguel Nobre Parreira Cacho Teixeira
Analysis of 8 2-DE gel images, corresponding to 4 pairs of experimental replicates, using the Samespots software. Analysis included: spot detection, matching and quantification of the normalized intensity; statistical analysis of the results - PCA and clustering according to expression profile.
2-DE gel analysis using the Samespots software
21 novembro 2019, 09:30 • Miguel Nobre Parreira Cacho Teixeira
Analysis of 8 2-DE gel images, corresponding to 4 pairs of experimental replicates, using the Samespots software. Analysis included: spot detection, matching and quantification of the normalized intensity; statistical analysis of the results - PCA and clustering according to expression profile.
Metabolomics analysis
21 novembro 2019, 08:00 • Miguel Nobre Parreira Cacho Teixeira
Metabolomics: definition and principles. Critical analysis of sample preparation and metabolite extraction methods.Metabolomics analysis using MS approaches (GS-MS, LC-MS and DIMS) and NMR.
2-DE gel analysis using the Samespots software
20 novembro 2019, 12:30 • Miguel Nobre Parreira Cacho Teixeira
Analysis of 8 2-DE gel images, corresponding to 4 pairs of experimental replicates, using the Samespots software. Analysis included: spot detection, matching and quantification of the normalized intensity; statistical analysis of the results - PCA and clustering according to expression profile.
Non 2-DE Expression Proteomics
18 novembro 2019, 11:00 • Miguel Nobre Parreira Cacho Teixeira
Non 2-DE Expression Proteomics: MudPit. ICAT, iTRAQ and SILAC. LC-MS Proteomics from a chemometrics perspective, aiming improved diagnosis tools