Sumários

CW1: From high-throughput sequencing to a genome annotation (part 2)

8 março 2019, 13:30 • Nuno Gonçalo Pereira Mira

CW1: From high-throughput sequencing to a genome annotation: discussion on methodologies to examine variants of the same species at a genomic level. Detection of SNPs and larger genomic modifications. Basic concepts underlying automatic gene detection.

Third-generation sequencing technologies

7 março 2019, 10:00 • Nuno Gonçalo Pereira Mira

PacBio as a paradigm of third generation sequencing technologies. Advantages, limitations and applicability. 

Discussion on the usefulness of guided vs de novo assembly.  Basics on automatic gene detection and methods to validate informatic predictions

CW1: From high-throughput sequencing to a genome annotation (part 2)

6 março 2019, 12:30 • Nuno Gonçalo Pereira Mira

CW1: From high-throughput sequencing to a genome annotation: discussion on methodologies to examine variants of the same species at a genomic level. Detection of SNPs and larger genomic modifications. Basic concepts underlying automatic gene detection.

CW1: From high-throughput sequencing to genome annotation.

1 março 2019, 13:30 • Nuno Gonçalo Pereira Mira

CW1: From high-throughput sequencing to genome annotation. Discussion on the concepts of coverage and assembly

Basis on Illumina and pyrosequencing

28 fevereiro 2019, 10:00 • Nuno Gonçalo Pereira Mira

The library preparation step in Illumina (continuing from last class) and in pyrosequencing (bridge vs emulsion PCR). 

The sequencing step in Illumina (reversible termination) and in pyrosequencing. Limitations and advantages of each of the techniques.