Sumários
Biological Reactors - Stirring, mixing and oxygen transfer:
10 março 2016, 10:00 • Frederico Castelo Ferreira
Biological Reactors - Stirring, mixing and oxygen transfer: Microorganism diversity and environmental Conditions for microorganism’s growth. Oxygen Solubility in water. Mass Transfer and Movement of fluids: Mass transfer Definitions (Molecular Diffusion, Convective Mass Transfer, film theory, driving force, Fick law, diffusion coefficient, mass transfer coefficient ). Gas-Liquid film mass transfer. Partial pressure, dissolved oxygen and Henry law. Oxygen transfer. KLa concept and equation gas-liquid oxygen transfer equation. Examples of exercises
T1 - Penicillin Acylase production - Session II
9 março 2016, 10:30 • Frederico Castelo Ferreira
T1 - Session II: Start-up of a bioreactor with inoculum of Escherichia coliATCC 9637, cell growth monitoring of operating parameters and sampling of the culture medium. Determination of biomass. Shift 2
Biological Reactors - bioprocess stoichiometry and kinetics (cont)
8 março 2016, 11:00 • Frederico Castelo Ferreira
Biological Reactors - bioprocess stoichiometry and kinetics (cont) : Culture regimes (Batch, fedbatch, continuous with cell retention). Mass balance – Chemostat. Chemostat with cell retention, Chemostat with cell recycle. Mass balance – Two stage chemostat system. Examples of exercises
T1 - Penicillin Acylase production - Session I
7 março 2016, 10:00 • Frederico Castelo Ferreira
T1 - Penicillin Acylase production - Session I. Preparation of a bioreactor for controlled cell culture in batch. Shift 2