Publicação de Notas

20 junho 2015, 00:20 • Frederico Castelo Ferreira

Grades from first Exame. Exames will be available for consultation and will be a question session on Friday, June 27 at 10.00 in room t.b.a.

T3 Lab session - March 23 and 25, 2015

18 março 2015, 18:03 • Nídia Dana Lourenço

Dear students,

The updated protocol for lab session “T3 –Membrane Technology” is now available at the protocol section. Please read it carefully before next week’s lab session (March 23 and 25, 2015). Bring also the protocols T1 and T2 with you, since you will be quantifying the activity of PGA by the PDAB method (T1) and the total protein by the Bradford method (T2).

Looking forward to seeing you next week,

Nídia

Next week, March, 2-6 2015, planning

28 fevereiro 2015, 10:30 • Frederico Castelo Ferreira

Dear Students

This week, March 2-6, Group 1, 2 and 3 will work on the laboratory. We will have to do in this T1-Cell culture and T2-Cell rupture during this week (check fenix for the protocols).

Therefore we have to start straight working in the laboratory, Monday, March 2 at 10.00 AM (Please bring your lab coats Monday and read T1 before).

The workplan for the week will be (apologies for the change over the arrangements discussed in the class room):

-Monday, March 2^nd,10.00-13.00. Preparation of Media and Bioreactors (T1)

-Tuesday, March, 3^rd, 11.00-12.30 Rules on lab book writing and discussion on good lab practice and hints on report preparation.

-Wednesday, March 4^th, 10.30-13.30. Cell culture (T1)

-Thursday, March 5^th, 10.00-11.30m Cell Rupture (T2)

Many thanks

Frederico

PS. We did optimize the cell cultures, and this year, we use the following concentrations:

-Pre-inoculum: Yeast extract (YE ) 40 g/L; phenyl acetic acid (AFA)  1,0 g/L; pH 7,5

 -Culture media (YE) 10 g/L;  AFA 3,0 g/L;  pH 7,2

week of 23-27 Feb 2015

21 fevereiro 2015, 12:21 • Frederico Castelo Ferreira

Dear Students,

For the week of 23-27 Feb 2015 all the students attend the shift of Wednesday. Please study the protocols, which will be in the fenix monday early morning. The tuesday and thursday classes will be mantained in regular calendar.

Many thanks,
Frederico

Tomorrow class (18 Feb 2015) at PA2

17 fevereiro 2015, 09:11 • Frederico Castelo Ferreira

Dear Students

Tomorrow class of EB will be in PA2 , instead of the laboratory of the 7th floor.

Looking forward to meet you,

Many thanks

Frederico