Sumários
Protein engineering III
31 março 2020, 11:00 • Gabriel Monteiro
Protein engineering:
- Engineering of motor proteins (Cytoskeletal, Polymerization, Rotary and Nucleic acid motors).
- Zinc finger proteins.
- Optogenetics.Protein engineering II
30 março 2020, 09:30 • Gabriel Monteiro
Protein engineering:
- Fluorescent proteins. XFPs, photoactivatable and photoshiftable fluorescent proteins.
- Protein labelling for classic and super-resolution microscopy. Sensors for monitoring cellular signals based on fluorescent proteins. Fluorescent timers. Nano-lanterns.Protein engineering I
24 março 2020, 11:00 • Gabriel Monteiro
Protein engineering:
-Protein design and re-design. Rational design and directed evolution. Site-directed and diversity-oriented protein engineering. Directed Evolution in vitro and in vivo. Methods to create genetic diversity in vitro (error-prone PCR and DNA shuffling, etc). Combinatorial display techniques (phage, bacterial and yeast surface display) and cell-free display systems (ribosome and mRNA display; in vitro compartmentalization).