Biological Dynamics - Diffusion

4 abril 2018, 11:00 • Maria Teresa Ferreira Marques Pinheiro

microscopic theory of diffusion

Brownian motion. Fundamental diffusion equation Finding a solution for the Diffusion equation -

(see support files “diffusion and biased diffusion”)

Diffusion with drift – biased diffusion equation or Smoluchowski equation 

Diffusion and mobility.

Significance of diffusion in biological context

Biological Dynamics - Diffusion

22 março 2018, 15:30 • Maria Teresa Ferreira Marques Pinheiro

The role of diffusion in biological systems. Diffusing molecules in a concentration gradient

Diffusion – Macroscopic theory

Diffusive dynamics (First and second Fick’s law).

Atomic Force Microscopy

21 março 2018, 11:00 • Maria Teresa Ferreira Marques Pinheiro

AFM: Basic principles and modalities

Probing and imaging mechanical properties of molecules, entities and cells with AFM.

Examples of the important contribution brought by AFM to solve biophysical questions:, e.g., virus maturation; lipid rafts and visualization of the configuration of membrane pores

Optical tweezers – load application and force measurements

15 março 2018, 15:30 • Maria Teresa Ferreira Marques Pinheiro

Optical tweezers: instrumentation, calibration, performance and proof of concept. Example of paper presentation based on the work of  Lang et al. An Automated Two-Dimensional Optical Force Clamp for Single Molecule Studies, Biophys J, 2002;83:491

Cell mechanics: probing mechanical properties of biomolecules

14 março 2018, 11:00 • Maria Teresa Ferreira Marques Pinheiro

How to probe mechanical properties: optical and magnetic tweezers

See Bibliography referred in slides - the most important items are available in "biblio" section