Sumários

Determination of anions in drinking water by ion exchange chromatography

12 novembro 2021, 14:00 • Zita Martins

Determination of anions in drinking water by ion exchange chromatography

Theory - Gas Chromatography

11 novembro 2021, 16:00 • Zita Martins

Gas chromatography. Gas chromatograph: Sample injection system, Column chromatography (packed and capillary). Stationary phase. Oven. Column temperature control (isothermal and program). Detectors: TCD, FID, ECD and NPD. Exercise and resolution (gas chromatography and internal standard method). Comparison between GC and HPLC.

Theory - Introduction to Chromatography and Liquid Chromatography

8 novembro 2021, 14:00 • Zita Martins

Introduction to Chromatographic Methods. Stationary phase, mobile phase. Planar chromatography and column chromatography. Column types (packed and capillary). Chromatogram, theoretical plate (N) and distribution constant (Kc). Parameters in column chromatography. Distribution constant, retention factor, selectivity factor, resolution factor.  Optimization of column efficiency. van Deemter's equation; Multipath term A; Longitudinal Diffusion Term (B/u); Mass transfer term (C). Exercises 1 and 2 and respective resolutions.


High Performance Liquid Chromatography (HPLC). Components of an HPLC. Pump, injector, guard column, column, detectors. Types of HPLC separation: adsorption, partition, ion excchange, molecular exclusion, affinity.

Identificação dos dois componentes de uma mistura por espectrometria de massa

5 novembro 2021, 14:00 • Alexandra Maria Moita Antunes

Identificação dos dois componentes de uma mistura com base nas experiencia de ESI (+) e ESI (-) obtidas em full scan e em MS/MS


Aula invertida: estudos de metabolómica e proteómica

4 novembro 2021, 16:00 • Alexandra Maria Moita Antunes

O que é um estudo proteómica

 Diferença entre abordagens proteómicas: Top-down e Bottom-up. 
Como é realizada a identificação de proteínas em estudos de proteómica (fragmentação de peptidos e pesquisa em bases de dados)

O que é estudo de metabolómica e qual o protocolo experimental utilizado.
Quais as vantagens e desvantagens dos estudos de metabolómica baseados em RMN e MS