NGS DNA sequencing technologies II: the sequencing step

30 setembro 2015, 11:00 • Nuno Gonçalo Pereira Mira

Library amplification in Illumina: bridge PCR and cluster formation.

Sequencing in SoLiD, Pyrosequencing, Ion Torrent and Illumina: the experimental details and the concept underneath the sequencing step in each of the techniques.

In silico analysis of an ORF sequence

25 setembro 2015, 11:00 • Miguel Nobre Parreira Cacho Teixeira

Analysis of a new uncharacterized ORF from the human pathogen C. glabrata, aiming functional prediction: ORF finder, BLASTN and BLASTP, multiple sequence alignment, prediction of protein parameters and post-translational modification.

In silico analysis of an ORF sequence

24 setembro 2015, 09:30 • Miguel Nobre Parreira Cacho Teixeira

Analysis of a new uncharacterized ORF from the human pathogen C. glabrata, aiming functional prediction: ORF finder, BLASTN and BLASTP, multiple sequence alignment, prediction of protein parameters and post-translational modification.

Introduction to Next Generation Sequencing Technologies.

24 setembro 2015, 08:00 • Nuno Gonçalo Pereira Mira

Next-generation sequencing methodologies.

Comparison with Sanger-sequencing-based methods. 

Overview on the workflow involving amplification of DNA libraries and subsequent sequencing steps. 

Library amplification in SoLiD, Ion Torrent and Pyrosequencing: Emulsion PCR.

In silico analysis of an ORF sequence

23 setembro 2015, 12:30 • Miguel Nobre Parreira Cacho Teixeira

Analysis of a new uncharacterized ORF from the human pathogen C. glabrata, aiming functional prediction: ORF finder, BLASTN and BLASTP, multiple sequence alignment, prediction of protein parameters and post-translational modification.