RNomics practice

6 novembro 2014, 09:30 • Jorge Humberto Gomes Leitão

Computational practice on the use of specific sofware for the prediction of secondary structures and of mRNA targets of a given bacterial sRNA.

RNomics - part II

6 novembro 2014, 08:00 • Jorge Humberto Gomes Leitão

Bacterial regulatory non-coding RNAs (sRNAs). Features and mechanisms of action of bacterial sRNAs. Examples of databases and software available for bacterial and eukaryotic non-coding RNA bioinformatics studies. Examples of experimental strategies for the identification of eukaryotic and prokaryotic non-coding RNAs.

RNomics practice

5 novembro 2014, 12:30 • Jorge Humberto Gomes Leitão

Computational practice on the use of specific sofware for the prediction of secondary structures and of mRNA targets of a given bacterial sRNA.

RNomics - part I

5 novembro 2014, 11:00 • Jorge Humberto Gomes Leitão

The RNome of a cell: coding and non-coding RNAs.

Regulatory non-coding RNAs in eukaryotes. The classes of eukaryotic non-coding regulatory RNAs. Discussion of the importance of mcroRNAs (miRNAs) and their roles on human  diseases such as cancer, neurodegenerative diseases and others. Impact of regulatory RNAs on agriculture including crop yields, resistance to stresses and plagues.

Comparative genomics and gene and genome evolution

23 outubro 2014, 08:00 • Paulo Jorge Moura Pinto da Costa Dias

The genomics revolution and comparative genomics. The phylogenetic trees of Hemiascomycetes. Radiation of the Saccharomyces sensu strictu group. Habitat, domestication and hybrids derived from Saccharomyces sensu strictu species. Yeast comparative genomics. Yeast Gene Order Browser (YGOB). Reconstruction of the evolutionary history of the S. cerevisiae genes TPO2 and TPO3. Yeast and human genomes architecture.

Prevalence of gene duplication. Molecular origins of gene duplication. Gene duplication and its importance in the evolutionary process. Concepts of neofunctionalization, subfunctionalization and dosage selection. Life stages, mechanisms of retention and fate of gene pairs. Subfunctionalization of a galactose gene regulatory circuit in S. cerevisiae. The human hemoglobin gene family. Expansion of the Drug:H+ antiporter family 2 in the Hemiascomycetes.

The C-value paradox. Mechanisms of genome size change. “Selfish DNA” and the spread of transposable elements. “Junk DNA” and the accumulation of pseudogenes. Introns. Insertion-deletion biases.  Chromosome-level events. Polyploidy. Phylogenetic positions of some likely polyploidy events during eukaryote evolution. The 2R hypothesis. Evolutionary significance of ancient genome duplications.