Sumários
Metagenomics Hands-On Session
8 janeiro 2026, 14:00 • Tina Keller-Costa
Annotation, analysis and interpretation of secondary metabolite encoding biosynthetic gene clusters (BGCs), with AntiSMASH v. 8.0, using assembled metagemome data (6 samples) and two metagenome assembled genomes (MAGs) (and for comparison also a reference genome from a coral-derived bacterial isolate/type strain) obtained from the microbiomes of healthy and diseased coral and surrounding seawater samples.
Metagenomics Hands-On Session
7 janeiro 2026, 12:30 • Tina Keller-Costa
Annotation, analysis and interpretation of secondary metabolite encoding biosynthetic gene clusters (BGCs), with AntiSMASH v. 8.0, using assembled metagemome data (6 samples) and two metagenome assembled genomes (MAGs) (and for comparison also a reference genome from a coral-derived bacterial isolate/type strain) obtained from the microbiomes of healthy and diseased coral and surrounding seawater samples.
Metagenomics Hands-On Session
5 janeiro 2026, 14:00 • Tina Keller-Costa
Annotation, analysis and interpretation of secondary metabolite encoding biosynthetic gene clusters (BGCs), with AntiSMASH v. 8.0, using assembled metagemome data (6 samples) and two metagenome assembled genomes (MAGs) (and for comparison also a reference genome from a coral-derived bacterial isolate/type strain) obtained from the microbiomes of healthy and diseased coral and surrounding seawater samples.
Metagenomics Lecture
5 janeiro 2026, 11:00 • Tina Keller-Costa
The need for and history of Metagenomics tools in Microbial Ecology and Microbiome research was explained (The problem of microbial diversity and the great plate count anomaly etc). We looked at how metagenomics has reshaped our view on the Tree of Life between 1977 and 2016. Three core metagenomics workflows were explained in detail: (1) Amplicon (Metabarcoding) studies for microbial diversity analyses: the use as the 16S rRNA gene as marker for prokaryotic diversity studies, layout of a typical amplicon sequencing workflow and pipeline (OTUs vs ASVs; Qiime2/DADA2 pipeline) (2) Full shot-gun sequencing-based metagenomics for functional and taxonomic analyses and for the reconstruction of metagenome-assembled genomes (MAGs); typical pipelines and workflows, and metagenome assembly and binning tools and methods were introduced. (3) metagenomic libraries for bioexploration (screening for novel enzymes and natural products). Metagenomic case studies on the microbiomes of corals, marine sponges, and the human gut were presented and discussed with the class. Small Quiz questions were answered together with the class to consolidate the new knowledge.
RNOmics Practice
19 dezembro 2025, 15:00 • Jorge Humberto Gomes Leitão
Softwares dedicated to secondary structure prediction and mRNA target prediction were used to predict the secondary structure and the mRNA targets of the non-coding RNA RIT11b.