Sumários
Functional Genomics
26 abril 2018, 10:00 • Miguel Nobre Parreira Cacho Teixeira
Systematic functional analysis vs Functional Genomics. Other omics approaches: disruptome analysis; synthetic genetic arrays; localizomics.
Genome-wide data analysis using bioinformatics tools
24 abril 2018, 15:30 • Miguel Nobre Parreira Cacho Teixeira
Integrated transcriptome and disruptome data analysis using bioinformatics tools: GOToolBox, KEGG, YEASTRACT and STRING. The propionic acid case-study.
Non 2-DE Expression Proteomics and Genome-wide data analysis
24 abril 2018, 09:00 • Miguel Nobre Parreira Cacho Teixeira
Non 2-DE Expression Proteomics approaches: TAP western approach; MudPit; ICAT, iTRAQ and SILAC. LC-MS Proteomics from a chemometrics perspective, aiming improved diagnosis tools.
2-DE gel analysis using the Samespots software
13 abril 2018, 13:30 • Miguel Nobre Parreira Cacho Teixeira
Analysis of 8 2-DE gel images, corresponding to 4 pairs of experimental replicates, using the Samespots software. Analysis included: spot detection, matching and quantification of the normalized intensity; statistical analysis of the results - PCA and clustering according to expression profile.
Expression, phospho and redox proteomics
12 abril 2018, 10:00 • Miguel Nobre Parreira Cacho Teixeira
New approaches to 2-DE-based expression proteomics. Differencial Gel Electroforesis (DIGE). Global analysis of protein modifications: phospho and glycoproteomics; redox proteomics.