Sumários
Metabolic pathway modeling
2 maio 2017, 15:30 • Miguel Nobre Parreira Cacho Teixeira
Metabolic pathway modeling using the COPASI software. Mathematical description of biochemical pathways. Simulation of pathway behavior upon changes in enzyme kinetic parameters and upon changes in substrate concentrations.
Metabolomics analysis
2 maio 2017, 09:00 • Miguel Nobre Parreira Cacho Teixeira
Metabolomics: definition and principles. Critical analysis of sample preparation and metabolite extraction methods.Metabolomics analysis using MS approaches: GS-MS, LC-MS and DIMS. Metabolomics analysis based on NMR.Chemometrics approaches to NMR or MS data analysis: PCA and PLS-DA. Examples of application of metabolomics tools to the study of silent mutations, functional genomics and metabolic module definition.
Metabolomics data analysis
28 abril 2017, 13:30 • Miguel Nobre Parreira Cacho Teixeira
Metabolomics data analysis using the online tool MetaboAnalyst: statistical (Correlation analysis, PCA, PLS-DA and clustering) and pathway enrichment analysis. Biological interpretation of the data.
Functional Genomics (cont.)
27 abril 2017, 10:00 • Miguel Nobre Parreira Cacho Teixeira
Omics approaches for the study of protein-protein (genome-wide yeast two-hybrid system; protein chips), protein-DNA (ChIP-on-chip and ChIP-seq) and protein-ligand (protein chips) interactions.
Metabolomics data analysis
26 abril 2017, 12:30 • Miguel Nobre Parreira Cacho Teixeira
Metabolomics data analysis using the online tool MetaboAnalyst: statistical (Correlation analysis, PCA, PLS-DA and clustering) and pathway enrichment analysis. Biological interpretation of the data.