Students, as you know this week we will initiate a set of three lab classes that are focused on yeast production of bioethanol under very high gravity conditions. The first class takes place this next week (WEEK 4) and is in silico (classrooms available on Fénix) and the following two classes are on the BSRG class lab (6th floor, South Tower; in front of the elevator). The lab guides are now available. Note that, after the in silico class is finished you will have only 48h to let me know which are the strains your modelling predicts to behave better than the wild-type strain. We need this tight schedule because strains need to be taken from the deep freezer before being tested. Each group identifies 3 mutants. That identification is made HERE.

After the in silico class students submit a table with the results of the modelling (fluxes under the different conditions and also results of Optknock). That table will be used later on for the oral discussion of results. I strongly advice to submit the table as soon as possible but you will have 1 week to do it, after the lab is done.

Students, the results from the HPLC for the shifts of MON and TUE are available HERE.

As for the weights, you can find the results that were taken by Prof Joana HERE. To calcule the fermentation rate of each strain and to obtain the data for the calibration curves download HERE.  

Don't forget to come count your colonies for the different cultures you had studied. 

 

   TUESDAY CLASS