Sumários
FCS and FRAP (online class)
9 abril 2021, 17:00 • Fábio Monteiro Fernandes
Fluorescence Recovery After Photobleaching (FRAP). Fluorescence Correlation Spectroscopy (FCS).
FRET Microscopy and FLIM (online class)
6 abril 2021, 14:30 • Fábio Monteiro Fernandes
Förster Resonance Energy Transfer (FRET) Microscopy. FRET and Fluorescent Proteins. Fluorescence Lifetime Imaging (FLIM).
Fluorescence Microscopy
26 março 2021, 17:00 • Fábio Monteiro Fernandes
Basic Concepts of Fluorescence Microscopy. Widefield Microscopy. Laser Scanning Confocal Microscopy (LSCM). Spinning Disk Confocal Microscopy. Two-photon excitation Microscopy. Total Internal Reflection Fluorescence Microscope (TIRFM). Super-resolution Microscopy.
Fluorescence Spectroscopy (aula online)
23 março 2021, 14:30 • Fábio Monteiro Fernandes
Fundamentals of Fluorescence. Instrumentation. Probes and fluorophores. Time-resolved data in fluorescence. Fluorescence Quenching. Fluorescence Polarization. Förster resonance energy transfer (FRET).
Absorption, Scattering and Circular Dichroism Spectroscopy (aula online)
19 março 2021, 17:00 • Fábio Monteiro Fernandes
Light and electronic transition concepts. UV-VIS spectroscopy. Light scattering. Circular Dichroism. Secondary protein structure.