Sumários

FCS and FRAP (online class)

9 abril 2021, 17:00 Fábio Monteiro Fernandes

Fluorescence Recovery After Photobleaching (FRAP). Fluorescence Correlation Spectroscopy (FCS).


FRET Microscopy and FLIM (online class)

6 abril 2021, 14:30 Fábio Monteiro Fernandes

Förster Resonance Energy Transfer (FRET) Microscopy. FRET and Fluorescent Proteins. Fluorescence Lifetime Imaging (FLIM). 


Fluorescence Microscopy

26 março 2021, 17:00 Fábio Monteiro Fernandes

Basic Concepts of Fluorescence Microscopy. Widefield Microscopy. Laser Scanning Confocal Microscopy (LSCM). Spinning Disk Confocal Microscopy. Two-photon excitation Microscopy. Total Internal Reflection Fluorescence Microscope (TIRFM). Super-resolution Microscopy.


Fluorescence Spectroscopy (aula online)

23 março 2021, 14:30 Fábio Monteiro Fernandes

Fundamentals of Fluorescence. Instrumentation. Probes and fluorophores. Time-resolved data in fluorescence. Fluorescence Quenching. Fluorescence Polarization. Förster resonance energy transfer (FRET).


Absorption, Scattering and Circular Dichroism Spectroscopy (aula online)

19 março 2021, 17:00 Fábio Monteiro Fernandes

Light and electronic transition concepts. UV-VIS spectroscopy. Light scattering. Circular Dichroism. Secondary protein structure.