Sumários
Cryo-EM (online class)
27 abril 2021, 14:30 • Fábio Monteiro Fernandes
History of cryogenic electron microscopy (Cryo-EM). Advantages of EM for resolution. Vitrification of water. Single-Particle Reconstruction. Direct Electron Detectors. In-situ structures. Virus structures. Advantages and disadvantages of Cryo-EM for protein structure determination.
Electron Microscopy (online class)
23 abril 2021, 17:00 • Fábio Monteiro Fernandes
Dual nature of electrons. Limits of microscopy resolution. Comparison with optical microscopy. Transmission electron microscope (TEM). Components of a TEM microscope. Correction of spherical aberration artefacts. Limitations for imaging of biological samples. Sample Preparation. Negative and positive staining. Immunogold labeling. Scanning electron microscope (SEM). USe of SEM for element analysis.
Atomic force microscopy-based force spectroscopy - biological and biomedical applications. (online class)
20 abril 2021, 14:30 • Fábio Monteiro Fernandes
Atomic force microscopy-based force spectroscopy - biological and biomedical applications.
Invited seminar: Dr. Filomena Carvalho (Instituto de Medicina Molecular, Universidade de Lisboa)Atomic Force Microscopy (AFM) (online class)
16 abril 2021, 17:00 • Fábio Monteiro Fernandes
Basic Principles. Imaging of Lipids, proteins and nucleic acids. Data treatment. Imaging cells. Combining AFM with optical microscopy. AFM-based Force Spectroscopy. Force-Measurement cycle. Force-distance curves. Stiffness. Binding studies.
Invited seminar: Dr. Nuno C. Santos (Instituto de Medicina Molecular, Universidade de Lisboa)
Single-Molecule FRET (online class)
13 abril 2021, 14:30 • Fábio Monteiro Fernandes
Bulk vs single-molecule measurements. Total Internal Reflection Fluorescence (TIRF). Single-molecule FRET (smFRET). In-cell smFRET:.Example: Tau protein conformation.
Invited seminar: Dr. Ana M. Melo (IBB/IN) Universidade de Lisboa.