Dissertação
{en=Magnetic Micro- and Nano-Particles as a Tool for Cell Biology} {} EVALUATED
{pt=Research in magnetic micro- and nano- particles, functionalized or not, have led to numerous new applications in cell biology. These particles can allow for detection or localized actuation. Indeed, if a particle is linked to a specific molecule it might allow for localized detection of that molecule. On the other hand if a particle is connected to a given cellular structure it can be used for localized mechanical actuation. In this study we have designed a new technique in which both topographic and magnetic signals from nanoparticles bound to antibodies are detected by magnetic force microscopy, revealing insights into protein content and localization below the cell membrane. Additionally, magnetic micro/nano particles are also used to localize, and to perform a controlled actuation providing new insights into the mechanisms of cell stress response, e.g. those involving cytoskeleton. Therefore, we have developed a new experimental setup in which iron particles inside of cells are magnetically excited provoking a mechanical stress with controlled frequency and intensity. This system is intended for exploring different stress mechanisms, as mechanical stress and heat shock , en=Research in magnetic micro- and nano- particles, functionalized or not, have led to numerous new applications in cell biology. These particles can allow for detection or localized actuation. Indeed, if a particle is linked to a specific molecule it might allow for localized detection of that molecule. On the other hand if a particle is connected to a given cellular structure it can be used for localized mechanical actuation. In this study we have designed a new technique in which both topographic and magnetic signals from nanoparticles bound to antibodies are detected by magnetic force microscopy, revealing insights into protein content and localization below the cell membrane. Additionally, magnetic micro/nano particles are also used to localize, and to perform a controlled actuation providing new insights into the mechanisms of cell stress response, e.g. those involving cytoskeleton. Therefore, we have developed a new experimental setup in which iron particles inside of cells are magnetically excited provoking a mechanical stress with controlled frequency and intensity. This system is intended for exploring different stress mechanisms, as mechanical stress and heat shock }
novembro 9, 2007, 14:30
Orientação
CO-ORIENTADOR
Escola Superior de Tecnologia da Saúde de Lisboa
Professora Coordenadora
